Number of Credits
This course is about a common scenario in the transfusion service (TS) laboratory, the detection of anti-D at delivery in a female who has received Rh immune globulin (RhIg) during pregnancy. A case study is presented that will:
- Guide participants through laboratory findings that need to be interpreted and resolved;
- Examine current best practices in perinatal testing programs;
- Review the characteristics of RhIg and its use in pregnancy;
- Review and investigate key issues associated with detection of anti-D in women who have received antenatal RhIg;
- Discuss crossmatch and LIS policies related to RhIg-derived passive anti-D.
This case study presents an opportunity to review perinatal testing programs and the crucial role of RhIg in preventing hemolytic disease of the fetus and newborn (HDFN) due to anti-D. The case also examines practical aspects of routine serologic testing involving neonates and women who have received RhIg during pregnancy.
Intended Audience: This program is designed as an educational and training tool for Clinical laboratory technologists and technicians. This course is also appropriate for clinical laboratory science students and pathology residents.
- Discuss the history of RhIg, its sources, preparation, constituents, safety, types, mechanisms of action, criteria for the administration of RhIg antenatally and postnatally, dosage calculation, and causes of failures.
- Interpret serologic tests done on the mother, father, and fetus / newborn as part of perinatal testing programs, including antibody identification.
- Identify best practices for serologic and other tests used in perinatal testing programs.
- Describe the principles, uses, and limitations of the rosette test, Kleihauer-Betke test, and flow cytometry in Rh prevention programs.
- Discuss and resolve key issues associated with detection of anti-D in women who have received antenatal RhIg.
- Identify crossmatch and LIS policies and practices related to RhIg-derived passive anti-D.
This course has been approved for P.A.C.E.® credit. There are no prerequisites for this course.
- Case Scenario
- Perinatal Testing Programs
- Interpreting Initial Serologic Tests
- Review of Rh Immune Globulin
- Follow-up Serologic Tests
- Post-delivery Testing
- Summary and Conclusions
- Further Reading
- Graded Exam
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